Journal: Poultry Science

Article Title: Resveratrol protects against triclosan–induced impairment of colonic barrier function in chickens

doi: 10.1016/j.psj.2026.106946

Figure Lengend Snippet: Effects of RSV on mitochondrial function–related indicators exposed to TCS. (A, B) Protein expression and quantitative analysis of SIRT1, PGC–1α, NRF1, and mRNA expression levels (C) mRNA expression levels of SIRT1, PGC–1α, NRF1, and TFAM (D) mRNA expression levels of MFN2, OPA1, MFN1, DRP1, FIS1, and MFF. Data were analyzed using one–way ANOVA. Inter–group differences are indicated by different letters ( P < 0.05). Groups with the same letter were considered not to have significant differences (mean ± SD, n = 6).

Article Snippet: PGC-1α , 1:600 , WANLEIBIO.

Techniques: Expressing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Metformin drives HIF-1α–mediated dual metabolic reprogramming to enhance γδ T cell therapy in triple-negative breast cancer

doi: 10.1007/s00262-026-04351-w

Figure Lengend Snippet: Metformin exerts distinct effects on OXPHOS and glycolysis in γδ T cells versus TNBC cells through HIF-1α. A , B OCR profiles of TNBC cells and γδ T cells following 24-h metformin treatment, measured by Seahorse XF metabolic flux analysis. OCR curves were assessed under basal conditions and after adding the indicated mitochondrial inhibitors: oligomycin, FCCP, and rotenone/antimycin. C , D ECAR of TNBC cells and γδ T cells were quantified after 24 h of metformin exposure, with measurements taken during basal states and following sequential administration of glucose, oligomycin, and 2-DG. E Representative immunoblots showing AMPK signaling in TNBC and γδ T cells after 4 h exposure to increasing metformin doses. β-actin was employed for normalization. F , G OCR and ECAR profiles of γδ T cells following 24-h metformin and PX-478 treatment, measured by Seahorse XF metabolic flux analysis. All experimental procedures included a minimum of three independent biological replicates. Data represent mean ± SEM; statistical significance determined by two-tailed Student’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant. PX-478: HIF-1α Inhibitor

Article Snippet: The membrane was blocked with 5% non-fat milk in TBST (JXF003, Jingxin Bio) for 1 h at room temperature and subsequently incubated overnight at 4 °C with primary antibodies targeting phospho-AMPKα (2535 T, CST), AMPKα (2793S, CST), HIF-1α (2178S, CST), and β-actin (4970S, CST).

Techniques: Western Blot, Two Tailed Test